Mrs Athwal's husband Sukhdave Athwal, 43, and his mother, from Hayes, west London, both deny murder.
The court heard she was having an affair with a married man and had started divorce proceedings.
The prosecution alleges the mother and son arranged the murder because family honour was at stake.
'Culturally inappropriate'
Surjit Athwal's brother Jangdeesh Singh said her mother-in-law had asked him to persuade his sister against divorcing.
"She was very emphatic that it was over her dead body that this divorce would be allowed," he told the court.
"She said nothing about consequences but that it would be over her dead body that this divorce would be allowed."
Mr Singh said she told him divorce was considered "culturally inappropriate and unacceptable".
But he said his sister was "unhappy", "distressed" and "very much taking about divorce".
Mrs Athwal was accompanying her mother-in-law to India when she disappeared. Her body has never been found.
The trial continues.